Choline acetyltransferase and acetylcholinesterase: evidence for essential histidine residues.
نویسنده
چکیده
ABSTKACT: Choline acetyltransferase (EC 2.3.1.6) catalyzes the biosynthesis of acetylcholine according to the following chemical equation: acetyl coenzyme A + choline + acetylcholine + coenzyme A. Ethoxyformic anhydride inactivates the enzyme prepared from bovine brain. Acetyl coenzyme A and coenzyme A, but not choline or acetylcholine, substantially protect against inactivation. The enzyme is reactivated by hydroxylamine treatment. The apparent pK, of the reactive group is about 6.5. Ethoxyformic anhy-
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ورودعنوان ژورنال:
- Biochemistry
دوره 13 25 شماره
صفحات -
تاریخ انتشار 1974